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%D \module
%D   [      file=t-chromato,
%D        version=2008.04.05,
%D          title=\CONTEXT\ User Module,
%D       subtitle=Macros for chromatograms,
%D         author=Peter Münster,
%D           date=\currentdate,
%D      copyright={Peter Münster}]
%C This module is copyrighted by Peter Münster.
%C Please send any comments to pmrb at free.fr.

% This program is free software; you can redistribute it and/or
% modify it under the terms of the GNU General Public License
% as published by the Free Software Foundation; either version 2
% of the License, or (at your option) any later version.

% This program is distributed in the hope that it will be useful,
% but without any warranty; without even the implied warranty of
% merchantability or fitness for a particular purpose.  See the
% GNU General Public License for more details.

\startmodule[chromato]

\writestatus{loading}{Macros for chromatograms}

\unprotect

%D Some internal dimensions:
\doifundefined{Width}{\newdimen\Width}
\doifundefined{Height}{\newdimen\Height}

%D \macros{startChromato}
%D Starting a chromatogram.
\def\startChromato{\bgroup\dosingleempty\dostartChromato}
\def\dostartChromato[#1]#2#3{\getparameters[CHROM][dim=0.6,#1]
  \def\myChromFig{\doifdefinedelse{CHROMSdim}{%
    \externalfigure[#2][height=\CHROMSdim\textheight]}{%
    \externalfigure[#2][width=\CHROMdim\makeupwidth]}}%
  \setbox\scratchbox\hbox{\myChromFig}%
  \Width=\wd\scratchbox \Height=\ht\scratchbox \setuppositioning[unit=pt]
  \gdef\SavedCaption{#3}\doifundefined{CHROMSdim}{\placefigure[][fig:#2]{#3}}%
  \bgroup\startpositioning\position(0,0){\myChromFig}}

%D \macros{stopChromato}
%D End of chromatogram.
\def\stopChromato{\stoppositioning\egroup\egroup{\doifdefined{CHROMSdim}{%
      \SavedCaption}}}

%D \macros{startChromatos}
%D Begin set of chromatograms.
\def\startChromatos{\dosingleempty\dostartChromatos}
\def\dostartChromatos[#1]#2#3{\placefigure[][fig:#2]{#3}\bgroup
  \getparameters[CHROMS][dim=0.3,#1]
  \doifdefined{CHROMSdist}{\setupcombinations[distance=\CHROMSdist em]}%
  \startcombination[2*1]}

%D \macros{stopChromatos}
%D End of chromatograms.
\def\stopChromatos{\stopcombination\egroup}

%D Internal macro: position left or right:
\define[3]\LRposition{\edef\YPos{\withoutpt{\the\dimexpr(\withoutpt{%
        \the\dimexpr(#1pt/100)}\Height - 0.1\baselineskip)}}%
  \position(#2,\YPos){\vbox to 2pt{\vss{\hbox to 0pt{#3}\vss}}}}

%D \macros{Lbrace}
%D Place a brace at the left.
\define[4]\Lbrace{\LRposition{#1}0{\hss\rotate[rotation=90]{%
      $\overbrace{\hskip#2\Height}^{%
        \displaystyle\text{\rotate[rotation=270]{#4}}}$}\hskip#3em}}

%D \macros{Rbrace}
%D Place a brace at the right.
\define[4]\Rbrace{\LRposition{#1}{\withoutpt{\the\Width}}{\hskip#3em
    \rotate[rotation=270]{$\overbrace{\hskip#2\Height}^{%
        \displaystyle\text{\rotate[rotation=90]{#4}}}$}\hss}}

%D \macros{Larrow}
%D Place a flesh at the left.
\define[2]\Larrow{\LRposition{#1}0{\hss{\tx#2} $\rightarrow$}}

%D \macros{Rarrow}
%D Place a flesh at the right.
\define[2]\Rarrow{\LRposition{#1}{\withoutpt{\the\Width}}{$\leftarrow$ \tx#2}}

%D \macros{Above,Abrace}
%D Place something above the chromatogram.
\define[3]\Oposition{\edef\XPos{\withoutpt{\the\dimexpr(\withoutpt{%
      \the\dimexpr(#1pt/100)}\Width)}}%
    \position(\XPos,-\withoutpt{\the\dimexpr(#2\baselineskip)}){%
    \hbox to 0pt{\hss#3\hss}}}
\define[2]\Above{\Oposition{#1}{0.8}{\tx#2}}
\define[3]\Abrace{\Oposition{#1}{2.5}{%
    $\overbrace{\hskip#2\Width}^{\displaystyle\text{#3}}$}}

\protect

\stopmodule

\doifnotmode{demo}{\endinput}

%D Usage example:
\usemodule[chromato]
\setupexternalfigures[directory=samples]
\mainlanguage[de]
\starttext
\startChromato{ecolizeit}{Expression der rekombinanten Fucosyltransferase~V in
  {\em E.~coli}.}
  \Above{5}{BRS}\Above{15}{1h}\Above{26}{2h}\Above{38}{3h}\Above{50}{4h}
  \Above{61}{1h}\Above{72}{2h}\Above{83}{3h}\Above{94}{4h}
  \Larrow{12}{97\,kDa}\Larrow{20}{66\,kDa}
  \Larrow{32}{45\,kDa}\Larrow{52}{31\,kDa}
  \Lbrace{39}{0.75}{4}{A}
  \Lbrace{90}{0.16}{4}{B}
  \Abrace{32}{0.42}{löslich}
  \Abrace{77}{0.42}{unlöslich}
  \Rarrow{29}{FucT~V}\Rarrow{87}{FucT~V}
\stopChromato
\startChromatos[dim=0.17,dist=2]{saeuger}{Expression von rekombinanter
  Fucosyltransferase~V in unterschiedlichen Säugerzellinien.}
  \startChromato{saeuger}{A}
    \Above{26}{CHO}\Above{74}{HEK}
    \Larrow{4}{96\,kDa}\Larrow{37}{66\,kDa}\Larrow{81}{45\,kDa}
  \stopChromato
  \startChromato{saeugerred}{B}
    \Above{26}{CHO}\Above{74}{HEK}
    \Rarrow{81}{FucT~V}
  \stopChromato
\stopChromatos
\stoptext